Stress-responsive microRNAs in Populus.
Identifieur interne : 003802 ( Main/Exploration ); précédent : 003801; suivant : 003803Stress-responsive microRNAs in Populus.
Auteurs : Shanfa Lu [États-Unis] ; Ying-Hsuan Sun ; Vincent L. ChiangSource :
- The Plant journal : for cell and molecular biology [ 1365-313X ] ; 2008.
Descripteurs français
- KwdFr :
- Analyse de profil d'expression de gènes (MeSH), Basse température (MeSH), Clonage moléculaire (MeSH), Contrainte mécanique (MeSH), Données de séquences moléculaires (MeSH), Eau (physiologie), Gènes de plante (MeSH), Populus (génétique), Populus (physiologie), Régulation de l'expression des gènes au cours du développement (MeSH), Régulation de l'expression des gènes végétaux (MeSH), Salinité (MeSH), Séquence nucléotidique (MeSH), Séquençage par oligonucléotides en batterie (MeSH), Technique de Northern (MeSH), Température élevée (MeSH), microARN (métabolisme).
- MESH :
- génétique : Populus.
- métabolisme : microARN.
- physiologie : Eau, Populus.
- Analyse de profil d'expression de gènes, Basse température, Clonage moléculaire, Contrainte mécanique, Données de séquences moléculaires, Gènes de plante, Régulation de l'expression des gènes au cours du développement, Régulation de l'expression des gènes végétaux, Salinité, Séquence nucléotidique, Séquençage par oligonucléotides en batterie, Technique de Northern, Température élevée.
English descriptors
- KwdEn :
- Base Sequence (MeSH), Blotting, Northern (MeSH), Cloning, Molecular (MeSH), Cold Temperature (MeSH), Gene Expression Profiling (MeSH), Gene Expression Regulation, Developmental (MeSH), Gene Expression Regulation, Plant (MeSH), Genes, Plant (MeSH), Hot Temperature (MeSH), MicroRNAs (metabolism), Molecular Sequence Data (MeSH), Oligonucleotide Array Sequence Analysis (MeSH), Populus (genetics), Populus (physiology), Salinity (MeSH), Stress, Mechanical (MeSH), Water (physiology).
- MESH :
- chemical , metabolism : MicroRNAs.
- genetics : Populus.
- physiology : Populus, Water.
- Base Sequence, Blotting, Northern, Cloning, Molecular, Cold Temperature, Gene Expression Profiling, Gene Expression Regulation, Developmental, Gene Expression Regulation, Plant, Genes, Plant, Hot Temperature, Molecular Sequence Data, Oligonucleotide Array Sequence Analysis, Salinity, Stress, Mechanical.
Abstract
MicroRNAs (miRNAs), a group of small non-coding RNAs, have recently become the subject of intense study. They are a class of post-transcriptional negative regulators playing vital roles in plant development and growth. However, little is known about their regulatory roles in the responses of trees to the stressful environments incurred over their long-term growth. Here, we report the cloning of small RNAs from abiotic stressed tissues of Populus trichocarpa (Ptc) and the identification of 68 putative miRNA sequences that can be classified into 27 families based on sequence homology. Among them, nine families are novel, increasing the number of the known Ptc-miRNA families from 33 to 42. A total of 346 targets was predicted for the cloned Ptc-miRNAs using penalty scores of =2.5 for mismatched patterns in the miRNA:mRNA duplexes as the criterion. Six of the selected targets were validated experimentally. The expression of a majority of the novel miRNAs was altered in response to cold, heat, salt, dehydration, and mechanical stresses. Microarray analysis of known Ptc-miRNAs identified 19 additional cold stress-responsive Ptc-miRNAs from 14 miRNA gene families. Interestingly, we found that individual miRNAs of a family responded differentially to stress, which suggests that the members of a family may have different functions. These results reveal possible roles for miRNAs in the regulatory networks associated with the long-term growth of tree species and provide useful information for developing trees with a greater level of stress resistance.
DOI: 10.1111/j.1365-313X.2008.03497.x
PubMed: 18363789
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
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<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Base Sequence (MeSH)</term>
<term>Blotting, Northern (MeSH)</term>
<term>Cloning, Molecular (MeSH)</term>
<term>Cold Temperature (MeSH)</term>
<term>Gene Expression Profiling (MeSH)</term>
<term>Gene Expression Regulation, Developmental (MeSH)</term>
<term>Gene Expression Regulation, Plant (MeSH)</term>
<term>Genes, Plant (MeSH)</term>
<term>Hot Temperature (MeSH)</term>
<term>MicroRNAs (metabolism)</term>
<term>Molecular Sequence Data (MeSH)</term>
<term>Oligonucleotide Array Sequence Analysis (MeSH)</term>
<term>Populus (genetics)</term>
<term>Populus (physiology)</term>
<term>Salinity (MeSH)</term>
<term>Stress, Mechanical (MeSH)</term>
<term>Water (physiology)</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>Analyse de profil d'expression de gènes (MeSH)</term>
<term>Basse température (MeSH)</term>
<term>Clonage moléculaire (MeSH)</term>
<term>Contrainte mécanique (MeSH)</term>
<term>Données de séquences moléculaires (MeSH)</term>
<term>Eau (physiologie)</term>
<term>Gènes de plante (MeSH)</term>
<term>Populus (génétique)</term>
<term>Populus (physiologie)</term>
<term>Régulation de l'expression des gènes au cours du développement (MeSH)</term>
<term>Régulation de l'expression des gènes végétaux (MeSH)</term>
<term>Salinité (MeSH)</term>
<term>Séquence nucléotidique (MeSH)</term>
<term>Séquençage par oligonucléotides en batterie (MeSH)</term>
<term>Technique de Northern (MeSH)</term>
<term>Température élevée (MeSH)</term>
<term>microARN (métabolisme)</term>
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<keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Populus</term>
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<keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Populus</term>
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<keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>microARN</term>
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<keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Eau</term>
<term>Populus</term>
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<term>Molecular Sequence Data</term>
<term>Oligonucleotide Array Sequence Analysis</term>
<term>Salinity</term>
<term>Stress, Mechanical</term>
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<term>Basse température</term>
<term>Clonage moléculaire</term>
<term>Contrainte mécanique</term>
<term>Données de séquences moléculaires</term>
<term>Gènes de plante</term>
<term>Régulation de l'expression des gènes au cours du développement</term>
<term>Régulation de l'expression des gènes végétaux</term>
<term>Salinité</term>
<term>Séquence nucléotidique</term>
<term>Séquençage par oligonucléotides en batterie</term>
<term>Technique de Northern</term>
<term>Température élevée</term>
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<front><div type="abstract" xml:lang="en">MicroRNAs (miRNAs), a group of small non-coding RNAs, have recently become the subject of intense study. They are a class of post-transcriptional negative regulators playing vital roles in plant development and growth. However, little is known about their regulatory roles in the responses of trees to the stressful environments incurred over their long-term growth. Here, we report the cloning of small RNAs from abiotic stressed tissues of Populus trichocarpa (Ptc) and the identification of 68 putative miRNA sequences that can be classified into 27 families based on sequence homology. Among them, nine families are novel, increasing the number of the known Ptc-miRNA families from 33 to 42. A total of 346 targets was predicted for the cloned Ptc-miRNAs using penalty scores of =2.5 for mismatched patterns in the miRNA:mRNA duplexes as the criterion. Six of the selected targets were validated experimentally. The expression of a majority of the novel miRNAs was altered in response to cold, heat, salt, dehydration, and mechanical stresses. Microarray analysis of known Ptc-miRNAs identified 19 additional cold stress-responsive Ptc-miRNAs from 14 miRNA gene families. Interestingly, we found that individual miRNAs of a family responded differentially to stress, which suggests that the members of a family may have different functions. These results reveal possible roles for miRNAs in the regulatory networks associated with the long-term growth of tree species and provide useful information for developing trees with a greater level of stress resistance.</div>
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<Abstract><AbstractText>MicroRNAs (miRNAs), a group of small non-coding RNAs, have recently become the subject of intense study. They are a class of post-transcriptional negative regulators playing vital roles in plant development and growth. However, little is known about their regulatory roles in the responses of trees to the stressful environments incurred over their long-term growth. Here, we report the cloning of small RNAs from abiotic stressed tissues of Populus trichocarpa (Ptc) and the identification of 68 putative miRNA sequences that can be classified into 27 families based on sequence homology. Among them, nine families are novel, increasing the number of the known Ptc-miRNA families from 33 to 42. A total of 346 targets was predicted for the cloned Ptc-miRNAs using penalty scores of =2.5 for mismatched patterns in the miRNA:mRNA duplexes as the criterion. Six of the selected targets were validated experimentally. The expression of a majority of the novel miRNAs was altered in response to cold, heat, salt, dehydration, and mechanical stresses. Microarray analysis of known Ptc-miRNAs identified 19 additional cold stress-responsive Ptc-miRNAs from 14 miRNA gene families. Interestingly, we found that individual miRNAs of a family responded differentially to stress, which suggests that the members of a family may have different functions. These results reveal possible roles for miRNAs in the regulatory networks associated with the long-term growth of tree species and provide useful information for developing trees with a greater level of stress resistance.</AbstractText>
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